Orientation and function of the nuclear-centrosomal axis during cell migration

► Anterior NC axis orientation is observed in many, but not all migrating cells. ► MT organization, vesicle trafficking and nuclear movement are NC axis effectors. ► NC axis orientation requires separate positioning of the centrosome and the nucleus. ► Substrate topology and cell-cell junctions affect NC axis orientation. ► Proper NC axis orientation is functionally important for the migration of some cells.