Invadosome regulation by adhesion signaling

► Invadosome assembly is promoted by extracellular matrix signaling. ► Invadosomes sense the mechano-chemical properties of extracellular matrix. ► β1/β3 integrins cross-talk in invadosome is revealed by their spatial organization. ► Reinforcement of Src signaling by PKC stimulates invadosome assembly. ► Invadosomes facilitate invasion through 3D actinic architecture coupled with intense protein trafficking.