A cis-regulatory module upstream of deltaC regulated by Ntla and Tbx16 drives expression in the tailbud, presomitic mesoderm and somites

► Ntla and Tbx16 directly regulate deltaC expression. ► Ntla and Tbx16 bind at a cluster of T-box sites in a cis-regulatory module (CRM) upstream of deltaC. ► This CRM drives expression of a reporter in the tailbud, PSM and somites during somitogenesis. ► The T-box site cluster is required for activation of the deltaC CRM in vivo.