A large-scale RNAi screen identifies functional classes of genes shaping synaptic development and maintenance

► In vivo screen in Drosophila identifies novel players in synaptic development and maintenance. ► Gene ontology analysis identifies overrepresented GO terms for different phenotypes. ► Proteasome subunits and mitotic spindle organizers are overrepresented in synaptic apposition phenotype group. ► NudE knock down causes axonal transport and retractions and the phenotype is replicated by a classical genetic mutant.