Two novel EGFP insertion alleles reveal unique aspects of Pax2 function in embryonic and adult kidneys

► Two new Pax2 mutant alleles were created by EGFP insertion. ► A hypomorphic allele shows reduced Pax2 expression and structural kidney defects. ► Pax2 functions to maintain epithelial cell polarity in the intermediate mesoderm. ► The Pax2-EGFP alleles can facilitate studies in the kidney and nervous system.