Early B-cell factors 2 and 3 (EBF2/3) regulate early migration of Cajal-Retzius cells from the cortical hem

► Ebf2 is a novel marker for hem- and septum-derived Cajal-Retzius cells. ► Ebf2 mutant shows a transient migratory defect of hem-derived Cajal-Retzius cells. ► Ebf3 is expressed in similar territories as Ebf2 and rescues Ebf2 mutant phenotype. ► Both Ebf2 and Ebf3 are required for Cajal-Retzius cell migration.