Kruppel-like factor 5 is required for formation and differentiation of the bladder urothelium

► KLF5 is required for stratification and terminal differentiation of urothelial cells. ► Defects in urothelial maturation induces hydronephrosis. ► KLF5 is not required for maturation of the bladder neck and urethral epithelium. ► Grhl3 and Pparγ are candidate targets of KLF5 during urothelial maturation. ► KLF5 regulates paracrine interactions with bladder mesenchyme.