Wls-mediated Wnts differentially regulate distal limb patterning and tissue morphogenesis

► Mesenchymal Wntless mediates Wnt5a pathway to sustain distal limb outgrowth. ► Ectodermal Wnts are essential for maintaining distal mesenchymal progenitor identity. ► Ectodermal and mesenchymal Wnts oppositely regulate distal limb differentiation. ► Wls-mediated ectodermal Wnts are required for distal tendon/ligament induction.