Porcupine-mediated lipidation is required for Wnt recognition by Wls

► First comprehensive analysis of all Drosophila Wnt family members. ► Evidence for a functional link between Wls and Porcupine. ► Wnt Serine acylation is required for Wnt recognition by Wls. ► Secretion and signaling analysis of all glycosylation and palmitoylation sites in Wg.