ETS-dependent regulation of a distal Gata4 cardiac enhancer

► A distal enhancer of Gata4 is active in cardiac lineages in mouse embryos. ► Activity of the Gata4 cardiac enhancer restricts to the endocardium by E9.5. ► The Gata4 cardiac enhancer is dependent on four ETS binding sites. ► The Gata4 cardiac enhancer is bound by ETS factors Ets1 and Erg. ► We examine the expression of all ETS genes in the mouse embryonic heart at E8.5.