Neurovascular development in the embryonic zebrafish hindbrain

► Quantitative hindbrain vascular development characterization with cellular resolution. ► The hindbrain vasculature expands primarily via cell migration, not proliferation. ► Multi-stage characterization of the hindbrain's neurovascular anatomy. ► Major neuronal clusters and axons of the hindbrain unaffected by lack of endothelium. ► Endothelial absence impairs optic tectum and cerebellar morphogenesis.