Shroom3 and a Pitx2-N-cadherin pathway function cooperatively to generate asymmetric cell shape changes during gut morphogenesis

► Shroom3 directs gut morphogenesis via cell shape changes in the dorsal mesentery. ► Pitx2 regulates asymmetric N-cadherin expression to direct epithelial cell shape. ► Shroom3 and N-cadherin cooperatively regulate cell shape.