A regulatory 'landscape effect' over the HoxD cluster

► We generate a large inversion allele, on the centromeric side of the HoxD cluster. ► The centromeric landscape is flipped away, while leaving the cluster intact. ► This inversion causes shortening of both primary and secondary body axes. ► Mis-regulations of Hoxd genes underlie the observed phenotypes ► We discuss the importance of genomic “landscape effects” on gene regulation.