کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10932868 | 1093755 | 2010 | 14 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Caveolin-1 regulates dorsoventral patterning through direct interaction with β-catenin in zebrafish
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیولوژی سلول
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Caveolin-1 regulates dorsoventral patterning through direct interaction with β-catenin in zebrafish Caveolin-1 regulates dorsoventral patterning through direct interaction with β-catenin in zebrafish](/preview/png/10932868.png)
چکیده انگلیسی
Caveolin-1 (Cav-1) is the principal component of plasma membrane caveolae that negatively regulates a number of cellular signaling events including canonical Wnt signaling. Activation of the Wnt/β-catenin pathway is essential for dorsal organizer formation and specification in early vertebrate embryos, but it remains not well understood what controls dorsal activity of maternal β-catenin and how Cav-1 functions in zebrafish embryogenesis. Here, we report that Cav-1 is required for proper dorsoventral patterning in zebrafish. Both Wnt and BMP signals act coordinately to negatively control transcriptional expression of cav-1 during embryonic development. Ectopic expression of Cav-1α or -1β resulted in formation of typical ventralized embryos, whereas Cav-1 knockdown led to abnormal embryos with expanded expression of dorsal genes. Cav-1 overexpression disrupts the nuclear translocation of β-catenin through the interaction of its scaffolding domain with Cav-1 binding motif of β-catenin. This reciprocal interaction is necessary for the ventralizing activity of Cav-1. We have further demonstrated that human Cav-1 proteins have conserved ventralizing activity in zebrafish embryogenesis. Thus, maternally expressed zebrafish Cav-1 regulates dorsoventral patterning by limiting nuclear translocation of active β-catenin.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Developmental Biology - Volume 344, Issue 1, 1 August 2010, Pages 210-223
Journal: Developmental Biology - Volume 344, Issue 1, 1 August 2010, Pages 210-223
نویسندگان
Saijun Mo, Lu Wang, Qing Li, Jie Li, Yuanyuan Li, Victor J. Thannickal, Zongbin Cui,