کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10933929 1093874 2005 17 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A dominant negative form of Rac1 affects myogenesis of adult thoracic muscles in Drosophila
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
پیش نمایش صفحه اول مقاله
A dominant negative form of Rac1 affects myogenesis of adult thoracic muscles in Drosophila
چکیده انگلیسی
Blocking Rac1 function in precursors of the indirect flight muscle of Drosophila severely disrupts muscle formation. The DLM fibers that develop using larval scaffolds are reduced in number and fiber size, while the DVMs, which develop using founder cells, are mostly absent. These adult muscle phenotypes are in part due to a reduced myoblast pool present at the third larval instar. BrDU labeling studies indicated that this is primarily due to a reduction in proliferation. In addition, DVM myoblasts display altered morphology and are unable to segregate into primordia. This defect precedes the evident block in fusion. We also show that the recently described DVM founder cells can be labeled with 22C10 and β-3 tubulin, and that they are present under conditions of dominant negative Rac1N17 expression. Despite the presence of founder cells, DVM fiber formation is rarely observed. Although DLM myoblasts are able to segregate around their larval scaffolds, the pace of fusion is reduced and consequently there is a delay in DLM fiber formation. Thus, in addition to its well-established role in fusion, Rac1 is also involved in the regulation of myoblast proliferation and segregation during adult myogenesis. These are two new roles for Rac1 in Drosophila.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Developmental Biology - Volume 285, Issue 1, 1 September 2005, Pages 11-27
نویسندگان
, , , , , , , , ,