Application of the systematic “DAmP” approach to create a partially defective C. albicans mutant

► The ''Decreased Abundance by mRNA Perturbation” (DAmP) technique can be used to modify gene expression in Candida albicans. ► The sun41-DAmP strain has biofilm and cell wall sensitivity phenotypes intermediate to the wild type and deletion strains. ► SUN41 is required for biofilm formation in vivo.