The structure-function relationship of the Aspergillus fumigatuscyp51A L98H conversion by site-directed mutagenesis: The mechanism of L98H azole resistance

► In this study we show how the L98H substitution confers to MAR in Aspergillus fumigatus ► The capacity of the entry channels is narrowed and binding of azoles is obstructed. ► Site-directed mutagenesis correlate the TR/L98H mutations to azole resistance. ► Codon 98 is highly conserved and important for maintaining the CYP51A structure.