کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10939656 1095311 2005 18 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A genome-wide screen for Saccharomyces cerevisiae nonessential genes involved in mannosyl phosphate transfer to mannoprotein-linked oligosaccharides
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
پیش نمایش صفحه اول مقاله
A genome-wide screen for Saccharomyces cerevisiae nonessential genes involved in mannosyl phosphate transfer to mannoprotein-linked oligosaccharides
چکیده انگلیسی
A collection of haploid Saccharomyces cerevisiae deletion strains-both MAT a and α-was screened for mutants that exhibit low dye binding (ldb) phenotype. This phenotype has previously been associated with reduced incorporation of mannosyl phosphate groups into the mannoprotein-linked oligosaccharides. We identified 199 nonessential genes whose deletion resulted in a detectable ldb phenotype. They fell into diverse functional categories, including those involved in protein glycosylation, vacuolar function, intracellular transport, cytoskeleton organization, transcription, signal transduction, among others. The study extends the number of known genes that affect mannosyl phosphorylation of mannoprotein-linked oligosaccharides, and establishes a link with other relevant pathways in the cell, especially vacuolar function. We have assigned an LDB name to four uncharacterized ORFs identified in this study: YCL005W, LDB16; YDL146W, LDB17; YLL049W, LDB18; and YOR322C, LDB19.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Fungal Genetics and Biology - Volume 42, Issue 9, September 2005, Pages 773-790
نویسندگان
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