کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10939759 | 1095330 | 2005 | 11 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Isolation and characterization of the Aspergillus nidulans eglC gene encoding a putative β-1,3-endoglucanase
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیولوژی سلول
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چکیده انگلیسی
The Aspergillus nidulans eglC gene, which encodes a putative β-1,3-endoglucanase, was isolated from a chromosome-specific library by using an expressed sequence tag, esd0113. The EglC open reading frame encodes a 465 amino acid polypeptide, of which the amino acid sequence showed 46% similarity to that of Saccharomyces cerevisiae β-1,3-endoglucanase. The eglC transcript level at the early stages of asexual and sexual developments was dependent on the presence of the nsdD gene that encodes a GATA-type transcription factor, confirming that the nsdD gene is necessary for full accumulation of the eglC transcript. Deletion of the eglC gene did not affect the radial growth rate, the germination rate of conidia, and both of asexual and sexual development. However, deletion of the gene led to hyphae more resistant to a cell wall-lyzing enzyme, implying that the cell wall structure of the eglC-null mutant is altered from a wild type one. Furthermore, deletion of the fadA and sfaD genes, that encode a Gα and a Gβ subunits of a heterotrimeric G protein, respectively, did not affect the eglC transcript level at the early developmental stages. In contrast, deletion of the flbA gene, that codes for a regulatory protein having an RGS (regulator of G protein signaling) motif, led to decrease in the eglC transcript level. The eglC transcript level was not higher in a creA mutant than in a wild type, indicating that the eglC gene is not sensitive to carbon-catabolite repression.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Fungal Genetics and Biology - Volume 42, Issue 7, July 2005, Pages 590-600
Journal: Fungal Genetics and Biology - Volume 42, Issue 7, July 2005, Pages 590-600
نویسندگان
Chang-Jun Choi, Hee-Jeong Ju, Byung-Hyun Park, Rui Qin, Kwang-Yeop Jahng, Dong-Min Han, Keon-Sang Chae,