کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10953 710 2007 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The construction of 3D-engineered tissues composed of cells and extracellular matrices by hydrogel template approach
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
The construction of 3D-engineered tissues composed of cells and extracellular matrices by hydrogel template approach
چکیده انگلیسی

The three-dimensional (3D)-engineered tissues composed of only cells and extracellular matrices (ECM) were constructed by the hydrogel template approach. The disulfide-crosslinked poly(γ-glutamic acid) hydrogels were prepared as a template hydrogel. These template hydrogels were easily decomposed under physiological conditions using reductants such as cysteine, glutathione and dithiothreitol by cleavage of disulfide crosslinkage to thiol groups. The decomposed polymers are soluble in cell culture medium. The cleaving of disulfide bond was determined by UV–vis and FT-IR spectroscopies. We successfully prepared the 3D-engineered tissues (thickness/diameter, 2 mm/1 cm) composed of mouse L929 fibroblast cells and ECM by the decomposition of only the template hydrogel with cysteine after 10 days 3D-cell culture on/in the template hydrogel. The size and thickness of the 3D-engineered tissues was completely transferred from the template hydrogel. The cultured L929 cells viability in the obtained engineered tissues was confirmed by a culture test, WST-1 method and LIVE/DEAD staining assay. The engineered tissue was self-standing and highly dense composite of the cultured cells and collagen produced by the cells. This hydrogel template approach may be useful as a new class of soft-tissue engineering technology to substitute a synthetic polymer scaffold to the ECM scaffold produced from the cultured cells.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biomaterials - Volume 28, Issue 17, June 2007, Pages 2729–2737
نویسندگان
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