A phenotypic screen to identify hypertrophy-modulating microRNAs in primary cardiomyocytes

► Here we describe the development of an automated screening assay to determine cardiomyocyte cell size. ► We used this assay to screen 230 miRNAs for their hypertrophy-modulating potential. ► Thereby we identified major hypertrophy-modulating miRNAs. ► Among them are several miRNAs that have previously not been involved in cardiomyocyte biology.