Functional and morphological preservation of adult ventricular myocytes in culture by sub-micromolar cytochalasin D supplement

► Cytochalasin D at 0.5 μM preserves morphology and function of cardiomyocytes in culture. ► Actin remodelling precedes T-tubular remodelling in ventricular myocytes. ► Cytochalasin D can act as a pharmacological tool to study cardiomyocyte remodelling.