Loss of interstitial collagen causes structural and functional alterations of cardiomyocyte subsarcolemmal mitochondria in acute volume overload

►Loss of interstitial collagen is a hallmark of volume overload (VO). ►Acute VO generates oxidative stress in rat left ventricle (LV). ►Acute VO results in LV subsarcolemmal mitochondrial (SSM) dysfunction. ►Observed degradation of SSM in acute VO is reversed by MMP inhibitor.