کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10956196 | 1098785 | 2014 | 12 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Real-time trafficking and signaling of the glucagon-like peptide-1 receptor
ترجمه فارسی عنوان
قاچاق واقعی در زمان واقعی و سیگنال گیری از گیرنده پپتید-1 مانند گلوکاگون
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیولوژی سلول
چکیده انگلیسی
The glucagon-like peptide-1 incretin receptor (GLP-1R) of family B G protein-coupled receptors (GPCRs) is a major drug target in type-2-diabetes due to its regulatory effect on post-prandial blood-glucose levels. The mechanism(s) controlling GLP-1R mediated signaling are far from fully understood. A fundamental mechanism controlling the signaling capacity of GPCRs is the post-endocytic trafficking of receptors between recycling and degradative fates. Here, we combined microscopy with novel real-time assays to monitor both receptor trafficking and signaling in living cells. We find that the human GLP-1R internalizes rapidly and with similar kinetics in response to equipotent concentrations of GLP-1 and the stable GLP-1 analogues exendin-4 and liraglutide. Receptor internalization was confirmed in mouse pancreatic islets. GLP-1R is shown to be a recycling receptor with faster recycling rates mediated by GLP-1 as compared to exendin-4 and liraglutide. Furthermore, a prolonged cycling of ligand-activated GLP-1Rs was observed and is suggested to be correlated with a prolonged cAMP signal.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular and Cellular Endocrinology - Volume 382, Issue 2, 15 February 2014, Pages 938-949
Journal: Molecular and Cellular Endocrinology - Volume 382, Issue 2, 15 February 2014, Pages 938-949
نویسندگان
Sarah Noerklit Roed, Pernille Wismann, Christina Rye Underwood, Nikolaj Kulahin, Helle Iversen, Karen Arevad Cappelen, Lauge Schäffer, Janne Lehtonen, Jacob Hecksher-Soerensen, Anna Secher, Jesper Mosolff Mathiesen, Hans Bräuner-Osborne,