Estrogens promote proliferation of the seminoma-like TCam-2 cell line through a GPER-dependent ERα36 induction

► Both estradiol and testosterone can stimulate TCam-2 seminoma cell proliferation. ► Estradiol can activate GPER-cAMP/PKA signaling pathway. ► TCam-2 cells can express ERα36, a truncated isoform of the ERα receptor. ► ERα36 expression is rapidly induced after estrogen treatment in a GPER-dependent manner. ► ERα36 is required for estradiol-dependent EGFR expression.