کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10956695 | 1099398 | 2010 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Binding of αII spectrin to 14-3-3β is involved in NCAM-dependent neurite outgrowth
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیولوژی سلول
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![عکس صفحه اول مقاله: Binding of αII spectrin to 14-3-3β is involved in NCAM-dependent neurite outgrowth Binding of αII spectrin to 14-3-3β is involved in NCAM-dependent neurite outgrowth](/preview/png/10956695.png)
چکیده انگلیسی
Members of the 14-3-3 protein family have been implicated in neuronal migration, synaptic plasticity and learning. Using affinity chromatography followed by mass spectrometry analysis, we show here that the cytoskeletal protein αII spectrin is a novel ligand of 14-3-3β. We found that 14-3-3β interacts with αII spectrin via the mode 2 14-3-3 binding motif RLIQS1302HP. Binding required phosphorylation of Ser1302 by casein kinase II and was enhanced in the presence of calmodulin. Co-immunoprecipitation of αII spectrin and 14-3-3β with the neural cell adhesion molecule NCAM suggested that the 14-3-3-spectrin-interaction affects NCAM function. Indeed, disruption of the 14-3-3β/αII spectrin interaction by mutating Ser1302 to Ala enhanced NCAM-dependent neurite outgrowth. Our results indicate that the phosphorylation-dependent interaction between 14-3-3β and αII spectrin acts as a switch between positive and negative regulation of neurite outgrowth stimulated by NCAM, representing a novel and acute mechanism preventing uncontrolled elongation of neuronal processes.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular and Cellular Neuroscience - Volume 45, Issue 1, September 2010, Pages 66-74
Journal: Molecular and Cellular Neuroscience - Volume 45, Issue 1, September 2010, Pages 66-74
نویسندگان
Elisa M. Ramser, Friedrich Buck, Melitta Schachner, Thomas Tilling,