کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10956719 | 1099416 | 2009 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Syndapin is dispensable for synaptic vesicle endocytosis at the Drosophila larval neuromuscular junction
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موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیولوژی سلول
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Syndapin is dispensable for synaptic vesicle endocytosis at the Drosophila larval neuromuscular junction Syndapin is dispensable for synaptic vesicle endocytosis at the Drosophila larval neuromuscular junction](/preview/png/10956719.png)
چکیده انگلیسی
Syndapin is a conserved dynamin-binding protein, with predicted function in synaptic-vesicle endocytosis. Here, we combine genetic mutational analysis with in vivo cell biological assays to ask whether Drosophila syndapin (Synd) is an essential component of synaptic-vesicle recycling. The only isoform of Drosophila syndapin (synd) is broadly expressed and at high levels in the nervous system. synd mutants are late-larval lethals, but fertile adult “escapers” frequently emerge. Contrary to expectation, we report that the Synd protein is predominantly postsynaptic, undetectable at presynaptic varicosities at Drosophila third-instar larval neuromuscular junctions. Electrophysiological and synaptopHluorin imaging in control, synd-deficient or synd-overexpressing motor neurons reveals that synd is dispensable for synaptic-vesicle endocytosis. Our work in Drosophila leads to the suggestion that syndapin may not be a general or essential component in dynamin-dependent synaptic-vesicle endocytosis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular and Cellular Neuroscience - Volume 40, Issue 2, February 2009, Pages 234-241
Journal: Molecular and Cellular Neuroscience - Volume 40, Issue 2, February 2009, Pages 234-241
نویسندگان
Vimlesh Kumar, Suneel Reddy Alla, K.S. Krishnan, Mani Ramaswami,