کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10956840 | 1099459 | 2005 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Accumulation of NaV1 mRNAs at differentiating postsynaptic sites in rat soleus muscles
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موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیولوژی سلول
پیش نمایش صفحه اول مقاله
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چکیده انگلیسی
Acetylcholine receptors (AChRs) and voltage-gated sodium channels (NaV1s) accumulate at different times in the development of the murine neuromuscular junction (NMJ). We used in situ hybridization to study the relationship of NaV1 mRNA accumulation to this difference. mRNAs encoding both muscle NaV1 isoforms, Nav1.4 and Nav1.5, were first concentrated at NMJs at birth, when the proteins start to accumulate. Within 4 weeks, Nav1.4 mRNA increased 5-fold at the NMJ while Nav1.5 mRNA became undetectable. NaV1 mRNA accumulation occurred even if the nerve was cut at birth. Like AChR mRNA, NaV1 mRNA accumulated at denervated synaptic sites on regenerating muscles and in response to ectopically expressed neural agrin. Clustering of NaV1 at the NMJ follows that of its mRNA while AChR clustering precedes its mRNA clustering by several days. This suggests that factors other than local mRNA upregulation determine the timing of clustering of these two important postsynaptic ion channels.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular and Cellular Neuroscience - Volume 28, Issue 4, April 2005, Pages 694-702
Journal: Molecular and Cellular Neuroscience - Volume 28, Issue 4, April 2005, Pages 694-702
نویسندگان
Mark A. Stocksley, Suad S. Awad, Carol Young, Robert N. Lightowlers, Hans-Rudolf Brenner, Clarke R. Slater,