کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10957001 | 1099470 | 2005 | 13 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
β-Amyloid (Aβ) causes detachment of N1E-115 neuroblastoma cells by acting as a scaffold for cell-associated plasminogen activation
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موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیولوژی سلول
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: β-Amyloid (Aβ) causes detachment of N1E-115 neuroblastoma cells by acting as a scaffold for cell-associated plasminogen activation β-Amyloid (Aβ) causes detachment of N1E-115 neuroblastoma cells by acting as a scaffold for cell-associated plasminogen activation](/preview/png/10957001.png)
چکیده انگلیسی
A major component of neuritic plaques in brain tissue of Alzheimer's disease patients is the β-amyloid peptide (Aβ). Accumulation of Aβ has been associated with increased neuronal cell death and cognitive decline. We have previously shown that amyloid peptides like Aβ bind tissue-type plasminogen activator (tPA) and stimulate plasmin production. Here we investigated how Aβ regulates plasmin formation by N1E-115 neuroblastoma cells and the effects of Aβ-mediated plasmin formation on cell attachment and cell survival. We find that Aβ induces excessive cell-associated plasmin generation that causes cell detachment. Cell detachment is inhibited by carboxypeptidase B (CPB), an enzyme that blocks plasmin formation by cleaving off C-terminal lysine residues. Plasmin and CPB control Aβ-induced cell detachment independently of direct effects on cell viability. Aβ40 as well as oligomeric and fibrillar forms of Aβ42 stimulated tPA-mediated plasminogen activation and cell detachment. Our results suggest that plasmin-mediated cell detachment could contribute to the pathological effects of Aβ in diseased brain.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular and Cellular Neuroscience - Volume 28, Issue 3, March 2005, Pages 496-508
Journal: Molecular and Cellular Neuroscience - Volume 28, Issue 3, March 2005, Pages 496-508
نویسندگان
Onno Kranenburg, Barend Bouma, Yoony Y.J. Gent, Colinda J. Aarsman, Rakez Kayed, George Posthuma, Bettina Schiks, Emile E. Voest, Martijn F.B.G. Gebbink,