کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
10957675 1099600 2015 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
DNA-based identification of Peucedanum ostruthium specimens and detection of common adulterants by high-resolution melting curve analysis
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیولوژی سلول
پیش نمایش صفحه اول مقاله
DNA-based identification of Peucedanum ostruthium specimens and detection of common adulterants by high-resolution melting curve analysis
چکیده انگلیسی
Masterwort (Peucedanum ostruthium, syn. Imperatoria ostruthium, Apiaceae) is an old economic plant in Alpine countries cultivated as ornamental plant and used for spirits and in folk medicine. P. ostruthium is a species that has often been confused with related Apiaceae species or morphologically similar roots or tubers resulting in products of minor quality. Masterwort can be distinguished from other Apiaceae species by nrDNA (ITS1 and ITS2). The analysed chloroplast markers (trnK 5′ intron, trnT-trnL, and psbA-trnH), however, showed no species-specific mutations. With the application of two primer pairs amplifying parts of ITS and developed for high-resolution melting curve analysis (HRM) the target species was distinguishable from the other Peucedanum and Apiaceae species of our reference set. A multiplex PCR/HRM was developed to detect adulterations with Gentiana spp., Aconitum napellus and Veratrum album.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular and Cellular Probes - Volume 29, Issue 6, December 2015, Pages 343-350
نویسندگان
, , ,