کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10957755 | 1099653 | 2005 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Detection and quantification of Shiga toxin-encoding genes in sheep faeces by real-time PCR
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیولوژی سلول
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Sheep faeces may be an important source of Shiga toxin (Stx)-producing Escherichia coli. We have, therefore, established and evaluated a real-time 5â²-nuclease PCR assay to quantify the stx1 and stx2 genes in sheep faeces. The detection limit of our assay for both stx1 and stx2 in spiked samples corresponded to 102-103Â CFU/g, which is lower than for other assays for measuring these genes in faecal samples. Quantification values for our assay ranged from 102 to 107Â CFU/g faeces. The assay was evaluated on native, unspiked faeces. All sheep tested (n=7) shed stx1, and the quantitative results corresponded to the gene copies in 103-104Â CFU/g. The level of stx2, however, was below the quantitative detection limit in all the samples analyzed. This quantitative stx1 and stx2 assay may be important in assessing whether sheep harbouring Shiga toxin-producing bacteria represent a potential hazard to human health.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Molecular and Cellular Probes - Volume 19, Issue 5, October 2005, Pages 363-370
Journal: Molecular and Cellular Probes - Volume 19, Issue 5, October 2005, Pages 363-370
نویسندگان
Camilla Sekse, Asta Solberg, Anne Petersen, Knut Rudi, Yngvild Wasteson,