Regulation of ecto-5′-nucleotidase (CD73) in cultured cortical astrocytes by different inflammatory factors

► Regulation of astrocytic e-5NT by TNF-α, LPS, IFN-γ, Glu and H2O2 is investigated. ► LPS, IFN-γ, Glu and H2O2 decrease, whereas TNF-α increases e-5NT activity. ► LPS, IFN-γ and Glu down-regulate e-5NT, while H2O2 modulates its enzymatic efficiancy. ► TNF-α increases e-5NT protein abundance, without alteration in mRNA expression level.