کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10958355 | 1100014 | 2010 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Perivascular nerve fiber α-synuclein regulates contractility of mouse aorta: A link to autonomic dysfunction in Parkinson's disease
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیولوژی سلول
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Parkinson's disease and other neurodegenerative disorders associated to changes in α-synuclein often result in autonomic dysfunction, most of the time accompanied by abundant expression of this synaptic protein in peripheral autonomic neurons. Given that expression of α-synuclein in vascular elements has been previously reported, the present study was undertaken to determine whether α-synuclein directly participates in the regulation of vascular responsiveness. We detected by immunohistochemistry perivascular nerve fibers containing α-synuclein in the aorta of mice while aortic endothelial cells and muscular fibers themselves did not exhibit detectable levels of this protein. To assess the effect of α-synuclein on vascular reactivity, aortic ring preparations obtained from α-synuclein-deficient knockout mice and from transgenic mice overexpressing human wild-type α-synuclein under the control of the tyrosine hydroxylase-promoter were mounted and equilibrated in organ baths for isometric tension recording. Lack of α-synuclein did not modify the relaxant responses to the endothelium-dependent (acetylcholine) and -independent (sodium nitroprusside) vasodilators, but resulted in a greater than normal norepinephrine-induced vasoconstriction along with a lowered response to dopamine, suggesting potential presynaptic changes in dopamine and norepinephrine releases in knockout mice. Overexpression of α-synuclein in TH-positive fibers resulted in complex abnormal responses, characterized by lowered acetylcholine-induced relaxation and lowered norepinephrin-induced contraction. Taken together, our data show for the first time that α-synuclein is present in sympathetic fibers supplying the murine aorta and provide evidence that changes in α-synuclein levels in perivascular fibers play a physiological role in the regulation of vascular function.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Neurochemistry International - Volume 56, Issue 8, July 2010, Pages 991-998
Journal: Neurochemistry International - Volume 56, Issue 8, July 2010, Pages 991-998
نویسندگان
Vannina G. Marrachelli, Francisco J. Miranda, José A. AlabadÃ, Miguel Milán, Marifé Cano-Jaimez, Martina Kirstein, Enrique Alborch, Isabel Fariñas, Francisco Pérez-Sánchez,