کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
11003709 1462014 2018 21 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Electrodeposited Prussian Blue on carbon black modified disposable electrodes for direct enzyme-free H2O2 sensing in a Parkinson's disease in vitro model
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Electrodeposited Prussian Blue on carbon black modified disposable electrodes for direct enzyme-free H2O2 sensing in a Parkinson's disease in vitro model
چکیده انگلیسی
In this work, we present the combination of Carbon Black (CB) and electrodeposited Prussian Blue (PB) covered with a Nafion layer on Screen-Printed electrodes (CB/PB-SPE) used for non-enzymatic H2O2 sensing in Neuroblastoma cell line SH-SY5Y. These cells were challenged with 6-hidroxidopamine (6-OHDA) for modelling Parkinson's disease. The electrodes surface was investigated using Scanning Electron Microscopy (SEM) and electrochemically characterized, in terms of electroactivity and stability. Electrochemical sensing of H2O2 was carried out at very low potentials (−50 mV), with a LOD of 0.01 μM and linear range between 0.2 and 1000 μM, allowing interference-free detection of H2O2 in the selected cell culture. The H2O2 concentration was successfully monitored in an experimental model of Parkinson's disease. These results pave the way to a method for the continuous monitoring of H2O2 in culture medium for future studies of the role of H2O2 in Parkinson's disease.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Sensors and Actuators B: Chemical - Volume 275, 1 December 2018, Pages 402-408
نویسندگان
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