کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
11023136 | 1701343 | 2018 | 57 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Functional analysis of MoSnf7 in Magnaporthe oryzae
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیولوژی سلول
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چکیده انگلیسی
Snf7 is the core subunit protein of the yeast endosomal sorting complex required for transport (ESCRT) complex, which plays important roles in endocytosis and autophagy. In this study, we characterized MoSnf7 in Magnaporthe oryzae, a homolog of yeast Snf7, the core protein of ESCRT-III subcomplex. Like Snf7, MoSnf7 also localizes next to the vacuoles. Deletion of MoSNF7 resulted in significant decrease in vegetative growth and pathogenicity. Further analyses of ÎMosnf7 mutants showed that they were defective in endocytosis, sexual and asexual development, turgor pressure maintenance of appressorium at hyphal tips, and cell wall integrity. Additional assays for the localization and degradation of GFP-MoAtg8 in ÎMosnf7 mutants showed that they were defective in autophagy pathway. Based on the roles of yeast Snf7 in endocytosis and autophagy, we propose that the decreased vegetative growth and pathogenicity of ÎMosnf7 rice blast fungus M. oryzae, was partly due to the conservative roles of MoSnf7 in vesicle trafficking and autophagy pathway.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Fungal Genetics and Biology - Volume 121, December 2018, Pages 29-45
Journal: Fungal Genetics and Biology - Volume 121, December 2018, Pages 29-45
نویسندگان
Jie Cheng, Ziyi Yin, Zhengguang Zhang, Yongheng Liang,