کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
11025889 | 1666480 | 2019 | 42 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Calpain2 but not calpain1 mediated by calpastatin following glutamate-induced regulated necrosis in rat retinal neurons
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موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
بیولوژی سلول
پیش نمایش صفحه اول مقاله

چکیده انگلیسی
The purpose of this study is to investigate whether calpastatin (CAST) plays an important role in the regulated necrosis (RN) in rat retinal neurons under an excessive glutamate condition and furthermore to investigate whether this process is regulated by calapin1 and calpain2. In the present study, glutamate triggered CAST inhibition, calpain2 activation and retinal neuronal RN after injury. The application of CAST active peptide could provide protective effects against activated calpain2 mediated RN. However, the calpain1 activity was not changed in these processes. Finally, in vivo studies further confirmed the role of the CAST-calpain2 pathway in cellular RN in the rat retinal ganglion cell layer and inner nuclear layer after glutamate excitation. In addition, flash electroretinogram results provided evidence that the impaired visual function induced by glutamate could recover after CAST peptide treatment. This research indicated that excessive glutamate may lead to CAST inhibition and activated calpain2, but not calpain1 activation, resulting in RN.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Annals of Anatomy - Anatomischer Anzeiger - Volume 221, January 2019, Pages 57-67
Journal: Annals of Anatomy - Anatomischer Anzeiger - Volume 221, January 2019, Pages 57-67
نویسندگان
Shuchao Wang, Yanxia Huang, Yuhan Yan, Hongkang Zhou, Mi Wang, Lvshuang Liao, Zhen Wang, Dan Chen, Dan Ji, Xiaobo Xia, Fengxia Liu, Jufang Huang, Kun Xiong,