کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
11029931 | 1646409 | 2018 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Ligation-promoted hyperbranched rolling circle amplification enables ultrasensitive detection of microRNA in clinical specimens
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Ligation-promoted hyperbranched rolling circle amplification enables ultrasensitive detection of microRNA in clinical specimens Ligation-promoted hyperbranched rolling circle amplification enables ultrasensitive detection of microRNA in clinical specimens](/preview/png/11029931.png)
چکیده انگلیسی
MiRNA is reported as an important biomarker candidate and therapeutic target in a variety of diseases and cancers. However, ultrasensitive, straightforward, isothermal, low-cost and credible methods for the detection of miRNA from clinical specimens still remain challenges. Here, we establish an isothermal, highly sensitive and specific method for miRNA analysis based on a novel ligation-promoted hyperbranched rolling circle amplification (L-HRCA). This strategy exhibits 103-fold sensitivity enhancement compared to T4 ligase-assisted target-primer BRCA. The excellent specificity of L-HRCA allows for discriminating target miRNA from negative miRNAs. This method is applied to analyse the expression level of miR-21 in hepatocellular carcinoma. These results indicated that the L-HRCA strategy will become a promising tool to quantify miRNA in early clinical diagnostics.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Sensors and Actuators B: Chemical - Volume 277, 20 December 2018, Pages 634-639
Journal: Sensors and Actuators B: Chemical - Volume 277, 20 December 2018, Pages 634-639
نویسندگان
Xiaoe Zhang, Yinong Liu, Yiwen Yang, Junjie Huang, Haitao Wang, Zirui Zhu, Xi Wang, Pei Ma, Xin Zhou, Shaoru Wang, Xiang Zhou,