کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
11062 715 2007 13 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Influence of glass and polymer coatings on CHO cell morphology and adhesion
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Influence of glass and polymer coatings on CHO cell morphology and adhesion
چکیده انگلیسی

Successful development of cell-on-chip microsystems where living cells are deposited and grown in microfabricated structures is highly dependent on the control of cell/substrate interactions. In this study, several materials of interest were tested for CHO cell growth and morphology: (i) glass, fibronectin-, poly-l-lysine- and 3-aminopropyltriethoxysilane (APTES)—treated glass and UV/O3-modified PDMS coating on glass as well as (ii) silicon, poly-l-lysine-, APTES-, O2 plasma-treated and oxide-coated silicon. In addition, we quantitatively characterized cell adhesion to these substrates using a radial flow detachment assay. Lack of correlation between cell adhesion and cell morphology was systematically observed for all substrates. In particular, we show that PDMS coatings on glass can be finely tuned by UV/O3 treatment to enhance cell adhesion and induce elongated morphology. Moreover, we observed a low shear stress cell detachment mechanism on silicon oxide coatings on silicon wafers. It is therefore possible with these coatings to selectively influence either cell adhesion or morphology.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biomaterials - Volume 28, Issue 8, March 2007, Pages 1572–1584
نویسندگان
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