کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
11356 735 2006 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Practical application of a chromogenic FXIIa assay
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Practical application of a chromogenic FXIIa assay
چکیده انگلیسی

Autohydrolysis of blood factor XII (FXII+FXIIa→2FXIIa)(FXII+FXIIa→2FXIIa) is found to be a facile reaction in neat-buffer buffer solutions of FXII but an insignificant reaction in the presence of plasma proteins. Autohydrolysis causes a chromogenic assay for FXIIa in buffer solution to strongly deviate from the traditional plasma-coagulation assay. Autohydrolysis can be accommodated by performing chromogenic detection of FXIIa as a rate assay in swamping concentrations of FXII. Rate-assay results performed in this way are shown to be in analytical agreement with the plasma-coagulation assay. Autohydrolysis can be used as a means of amplifying FXIIa produced by contacting neat-buffer solutions of FXII with biomaterials, suggesting a route to highly sensitive measurement of biomaterial hemocompatibility.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biomaterials - Volume 27, Issue 28, October 2006, Pages 4840–4845
نویسندگان
, ,