کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1140678 | 956737 | 2011 | 11 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Determination of flux directions by thermodynamic network analysis: Computing informative metabolite pools Determination of flux directions by thermodynamic network analysis: Computing informative metabolite pools](/preview/png/1140678.png)
Network thermodynamics focuses on the energetic analysis of complex metabolic networks. The method connects free Gibbs energies, metabolite concentrations and flux directions by fundamental thermodynamic laws. Here, a new application of network thermodynamics is presented that identifies those metabolite pools that have to be measured in order to determine as many flux directions as possible. For a medium-scaled reaction network such informative metabolite pools are computed with an approach based on Monte Carlo sampling. It turns out that some reactions can be directed with only a few measurements whereas other reactions cannot be directed even with a complete data set. High connectivity in metabolic reaction networks in alliance with concentration ranges make it impossible to intuitively foresee such results. In particular, the impact of measurements of a special type of metabolites being involved in many reactions, so called energetic currency metabolites is investigated.
Journal: Mathematics and Computers in Simulation - Volume 82, Issue 3, November 2011, Pages 460–470