کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
11660 751 2006 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Tissue engineering of bone on micropatterned biodegradable polyester films
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی بیو مهندسی (مهندسی زیستی)
پیش نمایش صفحه اول مقاله
Tissue engineering of bone on micropatterned biodegradable polyester films
چکیده انگلیسی

In this study, the effect of cell alignment on proliferation and phenotype expression of rat bone marrow derived osteoblasts on micropatterned (MP) PHBV and P(l/d,l)LA films with 27 μm wide parallel microgrooves was investigated. Immobilization of fibrinogen (Fb) on film surface by adsorption increased hydrophilicity, while covalent immobilization decreased it. Amount of Fb immobilized was significantly higher upon covalent bonding (153.1±42.4 μg Fb/cm2) than when adsorbed (10.0±3.3 μg Fb/cm2). It was observed that the presence of MP did not influence cell proliferation in the long run. Osteoblasts on MP films with adsorbed (MP Fba) and covalently immobilized Fb (MP Fbi) aligned parallel to the groove axis with mean deviation angles of 10.59±23.47 and 29.02±33.03°, respectively, while on tissue culture polystyrene (TCP), on unpatterned films (UNP) and on UNP with adsorbed Fb (UNP Fba) alignment with an arbitrary axis was much higher: 46.66±24.98, 48.72±31.19, 47.74±27.29 degrees, respectively. Fb-free MP films were not effective in cell alignment, and clumps were formed. Cell alignment achieved on MP Fba films did not influence cell proliferation, but increased differentiation, as shown by ALP activity per cell and the evenness and the amount of calcium phosphate deposition. It was concluded that orientation of cells was influential on their differentiation and also, MP cell carriers with chemical cues on their surfaces are important in improving tissue repair.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biomaterials - Volume 27, Issue 6, February 2006, Pages 885–895
نویسندگان
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