کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1173362 961668 2011 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Local and global factors affecting RNA sequencing analysis
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Local and global factors affecting RNA sequencing analysis
چکیده انگلیسی

High-throughput RNA sequencing (RNA-seq) continues to provide unparalleled insight into transcriptome complexity. Now the “gold standard” for assessing global transcript levels, RNA-seq is poised to revolutionize our understanding of transcription and posttranscriptional regulation of RNA. Despite significant advantages over prior experimental strategies, RNA-seq is not without pitfalls. We have identified a number of confounding factors that significantly affect sequencing coverage. These include regional GC content, preferential sites of fragmentation, and read “pile-up” due to primer affinity and transcript end effects. Independent of cell type and laboratory, when ignored, these factors can bias analyses. Understanding the underlying principles responsible for producing these artifacts is key to recognizing both their presence and how their effects may be controlled. Here we outline the causes of and strategies to avoid several previously unreported complicating factors common to RNA-seq experiments.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 419, Issue 2, 15 December 2011, Pages 317–322
نویسندگان
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