کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1173469 1491402 2014 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The Na+ transport in gram-positive bacteria defect in the Mrp antiporter complex measured with 23Na nuclear magnetic resonance
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
The Na+ transport in gram-positive bacteria defect in the Mrp antiporter complex measured with 23Na nuclear magnetic resonance
چکیده انگلیسی

23Na nuclear magnetic resonance (NMR) has previously been used to monitor Na+ translocation across membranes in gram-negative bacteria and in various other organelles and liposomes using a membrane-impermeable shift reagent to resolve the signals resulting from internal and external Na+. In this work, the 23Na NMR method was adapted for measurements of internal Na+ concentration in the gram-positive bacterium Bacillus subtilis, with the aim of assessing the Na+ translocation activity of the Mrp (multiple resistance and pH) antiporter complex, a member of the cation proton antiporter-3 (CPA-3) family. The sodium-sensitive growth phenotype observed in a B. subtilis strain with the gene encoding MrpA deleted could indeed be correlated to the inability of this strain to maintain a lower internal Na+ concentration than an external one.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 445, 15 January 2014, Pages 80–86
نویسندگان
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