کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1173532 1491374 2015 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
On the use of aptamer microarrays as a platform for the exploration of human prothrombin/thrombin conversion
ترجمه فارسی عنوان
در مورد استفاده از میکروآرامهای آپتامر به عنوان یک پلتفرم برای کشف تبدیل پروترومبین / ترومبین انسان
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی

Microarrays are particular biosensors with multiple grafted probes that are generally used for parallel and simultaneous detection of various targets. In this study, we used microarrays with aptamer probes in order to follow up the different biomolecular interactions of a single enzyme, the thrombin protein, involved in the complex coagulation cascade. More precisely, thanks to label-free surface plasmon resonance imaging, we were able to monitor in real time an important step in the firing of the coagulation cascade in situ—the enzymatic transformation of prothrombin into thrombin, catalyzed by factor Xa. We were also able to appraise the influence of other biochemical factors and their corresponding inhibiting or enhancing behaviors on thrombin activation. Our study opens the door for the development of a complete microarray-based platform not only for the whole coagulation cascade analysis but also for novel drug screening assays in pharmacology.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 473, 15 March 2015, Pages 66–71
نویسندگان
, , , ,