کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1174147 961732 2011 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Identification of a polymeric β-cyclodextrin-binding peptide from a phage-displayed peptide library
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Identification of a polymeric β-cyclodextrin-binding peptide from a phage-displayed peptide library
چکیده انگلیسی

In this work, a phage-displayed peptide library was applied to identification of β-cyclodextrin (CD)-binding peptide tag, capable of being combined to target peptides or proteins in a homogeneous way by established methods such as peptide synthesis and the recombinant DNA technique. Four enriched sequences were obtained after five rounds of biopanning against polymeric β-CD beads. One of the sequences showed high binding affinity to β-CD beads with a dissociation constant of approximately 7 × 10–6 M. The β-CD-binding sequence was used for immobilization of a hepatitis C virus (HCV) antigenic peptide on β-CD beads. The functionalized β-CD beads were successfully used for immunoassay of anti-HCV antibody with a detection limit of 1 ng. These results demonstrate that the identified peptide sequence has the potential of being used as an affinity tag to β-CD-containing surfaces.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 415, Issue 1, 1 August 2011, Pages 46–51
نویسندگان
, ,