کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1174258 | 961738 | 2013 | 8 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Phosphoprotein staining for sodium dodecyl sulfate–polyacrylamide gel electrophoresis using fluorescent reagent morin hydrate Phosphoprotein staining for sodium dodecyl sulfate–polyacrylamide gel electrophoresis using fluorescent reagent morin hydrate](/preview/png/1174258.png)
A fluorescence-based stain with 3,5,7,2′,4′-pentahydroxyflavone (morin hydrate, MH) was designed to stain phosphoproteins in one-dimensional sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE). Al3+ was applied as a “fixed bridge,” providing an efficient energy transfer channel between phosphoprotein and MH, to produce a strong fluorescent complex for the determination of phosphoprotein. As little as 62.5 ng of α-casein (7 or 8 phosphates) and β-casein (5 phosphates), 125 ng of ovalbumin (2 phosphates), and κ-casein (1 phosphate) could be visualized with a wide linear dynamic range. In comparison with conventional methods, MH stain is a time-saving method that takes just 90 min. It also has good compatibility with routine protein stainings such as Coomassie Brilliant Blue R (CBBR) and SYPRO Ruby for total protein analysis.
Journal: Analytical Biochemistry - Volume 435, Issue 1, 1 April 2013, Pages 19–26