کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1174372 | 961747 | 2009 | 5 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Menadione-catalyzed luminol luminescent assay as a novel evaluation method of ethanol tolerance in yeast cells Menadione-catalyzed luminol luminescent assay as a novel evaluation method of ethanol tolerance in yeast cells](/preview/png/1174372.png)
In this study, ethanol inhibited the growth and glucose-induced proton release of yeast cells in a dose-dependent manner. On the other hand, ethanol tolerance of menadione-catalyzed luminol luminescence by yeast cells increased with increasing ethanol concentrations in the growth medium. The intracellular reduced-form nicotinamide adenine dinucleotide (NADH) concentration also increased with increasing ethanol concentrations in the medium and was enough to maintain constant menadione-catalyzed luminol luminescence. These facts suggest that the menadione-catalyzed luminol luminescent assay depending on a NADH:quinone reductase and NADH generation system is useful as a new evaluation assay for assessing the vitality of ethanol-stressed yeast cells, whereas the glucose-induced proton release assay is expected to be useful for the evaluation of cell growth under ethanol stress.
Journal: Analytical Biochemistry - Volume 385, Issue 1, 1 February 2009, Pages 115–119