کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1174494 961753 2008 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A comparison between the responses of neutral red and acridine orange: Acridine orange should be preferential and alternative to neutral red as a dye for the monitoring of contaminants by means of biological sensors
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
A comparison between the responses of neutral red and acridine orange: Acridine orange should be preferential and alternative to neutral red as a dye for the monitoring of contaminants by means of biological sensors
چکیده انگلیسی

The acridine orange (AO) and neutral red (NR) dyes, commonly used as probes to measure the internal pH in acidic vesicles, are compared in this article. The comparison between the two dyes (arising from calculations taking into account their analytical constants) illustrated that the use of AO is preferential to that of NR because the AO response is sensitive over the whole pH range between 4.0 and 7.4, whereas the NR response is effective only between pHs 4.0 and 6.0. In addition, it became evident from the mitochondrial respiration response that NR, unlike AO, is a protonophore. When taken into consideration, these two properties suggest that AO is more suitable than NR as an indicator of toxicity measurements in water samples because the environmental toxic compounds induce pH changes in the acidic vesicles of biological structures that are used as environmental biosensors.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 383, Issue 2, 15 December 2008, Pages 316–319
نویسندگان
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