کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1174520 961754 2011 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
An enzyme-linked assay for the rapid quantification of microRNAs based on the viral suppressor of RNA silencing protein p19
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
An enzyme-linked assay for the rapid quantification of microRNAs based on the viral suppressor of RNA silencing protein p19
چکیده انگلیسی

MicroRNAs (miRNAs) are endogenous posttranscriptional regulators found in all metazoa and play crucial roles in virtually all cellular processes. Their aberrant expression has been linked to several diseased states; therefore, techniques capable of sensitive and specific profiling of the miRNA milieu will have significant application in prognostics, diagnostics, and therapeutics. Here we present a method for rapid quantification of miRNA levels using p19, a tombusvirus-encoded suppressor of RNA interference with sequence-independent and size-selective affinity toward 19-bp RNA duplexes. We present a surface plasmon resonance (SPR)-based miRNA sensing method where RNA probes are immobilized on gold surfaces demonstrating p19’s utility in recognition of miRNA-bound probes. This allows detection of miRNAs in the low nanomolar range. To increase the sensitivity, a bead-based enzyme immunoassay was performed, and this technique displays a lower detection limit of 1 fmol and a linear dynamic range from 1 pmol to 1 fmol.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 412, Issue 2, 15 May 2011, Pages 165–172
نویسندگان
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