کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1174675 961763 2008 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Electrochemical assay for deoxyribonuclease I activity
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Electrochemical assay for deoxyribonuclease I activity
چکیده انگلیسی

A thiolated oligonucleotide having three ferrocenes was immobilized on a gold electrode through the sulfur–gold linkage. This electrode showed a current response based on the redox reaction of the ferrocene moieties and this response was decreased after treatment with deoxyribonuclease I (DNase I), suggesting the disappearance of the ferrocene moieties on the electrode by the DNase I digestion. A linear correlation between i0 and i, which are current peaks before and after DNase I treatment, respectively, was observed and this slope was decreased with increase in the amount of DNase I. No current decrease was observed in the presence of EDTA or RNase A instead of DNase I. These results suggested that the current decrease responded specifically to the amount of DNase I and this electrode could be used for an electrochemical DNase I assay. Under the optimum conditions of DNase I digestion at 37 °C for 30 min, a quantitative analysis could be achieved in the range of 10−4–10−2 units/μl of DNase I.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Analytical Biochemistry - Volume 381, Issue 2, 15 October 2008, Pages 233–239
نویسندگان
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